evalution of in vitro effect of flavonoids on human low-density lipoprotein carbamylation
نویسندگان
چکیده
the non-enzymatic carbamylation of low density lipoprotein (ldl) is a naturally occurring chemical modification of apolipoprotein b as a result of condensation between lysine residues and cyanate derived from urea. carbamylated ldl is poorly recognized by ldl receptors and initiates different processes that can be considered proatherogenic. thus, ldl carbamylation may contribute to the increased risk of atherosclerosis in patients with kidney failure. the objective of this study was to investigate in vitro effects of flavonoids on ldl carbamylation. ldl was isolated from plasma using ultracentrifuge technique with a single step discontinuous gradient. then, cyanate was added to ldl and ldl carbamylation level was estimated in absence and presence of flavonoids by a colorimetric method at 530 nm. the results of this study showed that a number of flavonoids including rutin, catechin, morin, myricetin, kaempferol, taxifolin, luteolin, naringin and quercetin decreased ldl carbamylation in a dose dependent manner. also, it was demonstrated that these nutrients decreased electrophoretic mobility of carbamylated ldl. based on the results obtained in this study, it is suggested that flavonoids are able to inhibit ldl carbamylation (probably by scavenging cyanate ions) and thus, may have a role in ameliorating atherosclerotic risk of patients with kidney failure.
منابع مشابه
Evalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation
The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL carbamylation may contribute to the increase...
متن کاملEvalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation
The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL carbamylation may contribute to the increase...
متن کاملEvalution of In Vitro Effect of Flavonoids on Human Low-Density Lipoprotein Carbamylation
The non-enzymatic carbamylation of low density lipoprotein (LDL) is a naturally occurring chemical modification of apolipoprotein B as a result of condensation between lysine residues and cyanate derived from urea. Carbamylated LDL is poorly recognized by LDL receptors and initiates different processes that can be considered proatherogenic. Thus, LDL carbamylation may contribute to the increase...
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Effect of Lycopene on Formation of Low Density Lipoprotein-Copper Complex in Copper Catalyzed Peroxidation of Low Density Lipoprotein, as in vitro Experiment
Background: A great deal of evidence has indicated that oxidatively modified LDL plays a critical role in the initiation and progression of atherosclerosis. Antioxidants that can prevent LDL oxidation may act as antiatherogens. Copper is a candidate for oxidizing LDL in atherosclerotic lesions. The binding of copper ions to LDL is usually thought to be a prerequisite for LDL oxidation by copper...
متن کاملIn Vitro Effect of ?-Tocopherol, Ascorbic Acid and Lycopene on Low Density Lipoprotein Glycation
Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on LDL glycatio...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of pharmaceutical researchجلد ۲۰۱۰، شماره ۱، صفحات ۶۷-۷۴
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023